StarBright Dyes Expanding Fluorophore Choice for Multiplexing

Data presented at Immunology 2024 demonstrated how the features of StarBright™ Dyes simplified building human and mouse no compensation panels. In another poster, we show how the expansion of StarBright Dye–conjugated antibodies to include veterinary targets allows for the generation of larger multiplexing panels for bovine, porcine, and canine samples. Data acquired on the ZE5 Cell Analyzer.

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StarBright Dyes – Simplifying Flow Cytometry with Eight Color No Compensation Panels

StarBright Dyes — Simplifying Flow Cytometry with Eight Color No Compensation Panels

The brightness and narrow excitation and emission profiles make StarBright Dyes ideal for including in no compensation panels. Example mouse and human panels are shown, along with data on how to use them as a backbone for larger panels.
 

Starbright Dyes – Expanding the Availability and Choice of Fluorophores to Improve Immunophenotyping Data in Veterinary Studies

StarBright Dyes — Expanding the Availability and Choice of Fluorophores to Improve Immunophenotyping Data in Veterinary Studies

New veterinary targets conjugated to StarBright Dyes allow the generation of highly resolved panels to identify major cell types from bovine, porcine, and canine peripheral blood.
 



High Stability of StarBright Dyes

Data presented at CYTO 2024 focused on the stability of StarBright Dyes. One poster explains the benefits of StarBright Yellow Dyes, another highlights the stability of the full range of all 32 StarBright Dyes.

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StarBright Yellow, 561 nm Excitable Dyes, Superior Alternative to PE and PE-Tandems with Improved Stability and Better Spectral Properties

StarBright Yellow, 561 nm Excitable Dyes, Superior Alternative to PE and PE-Tandems with Improved Stability and Better Spectral Properties

Antibodies conjugated to StarBright Yellow Dyes show improved spectral properties and stability compared to comparison fluorophores.
 

Improving Flow Cytometry Data with Starbright Dyes: Fluorophores with High Stability and Assay Compatibility

Improving Flow Cytometry Data with StarBright Dyes: Fluorophores with High Stability and Assay Compatibility

StarBright Dyes are highly stable when used in different staining buffers and fixatives. They exhibit the same or improved photostability maintaining a stable spectral profile.
 



StarBright Dyes are Ideal for Multiplexing in Flow Cytometry and Western Blotting

Presented at AACR 2024, the benefits of StarBright Dyes are demonstrated in different applications using data acquired on a ZE5 Cell Analyzer or ChemiDoc MP.

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StarBright Dyes; Fluorophores with superior performance for multiplexing in Flow Cytometry and Western Blotting.

StarBright Dyes: Fluorophores with Superior Performance for Multiplexing in Flow Cytometry and Western Blotting.

The benefits of using StarBright Dyes for multiplexing in both applications are highlighted, such as brightness and compatibility with all protocols.
 



Improving and Expanding Immunophenotyping Panels with StarBright Dyes

Presented at AAI 2023, the features of StarBright Dyes, are highlighted using data acquired on the ZE5 Cell Analyzer. Additionally, a 38-color human immunophenotyping panel demonstrates that using antibodies conjugated to StarBright Blue and StarBright Yellow Dyes can expand panel size in spectral flow cytometry.

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StarBright Dyes – Making Panel Design Easier for Everyone

StarBright Dyes – Making Panel Design Easier for Everyone

The benefits of using StarBright Dyes for multiplexing are highlighted, such as narrow excitation and emission profiles and premixing.
 

Expanding the Size of Multicolor Panels with Ease with New StarBright Blue and StarBright Yellow Dyes

Expanding the Size of Multicolor Panels with Ease with New StarBright Blue and StarBright Yellow Dyes

38-color spectral panel, with a low complexity score, using StarBright Dyes on a 5-laser spectral flow cytometer.
 




Improving Flow Cytometry Assays with StarBright Dyes

Several posters presented at CYTO 2023 demonstrate the benefits of antibodies conjugated to the StarBright Dye range in several flow cytometry applications. These include large immunophenotyping panels, identifying rare cell populations, and using tetramers to detect antigen-specific T cells.

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StarBright Dyes – Improved Panel Design with New StarBright Blue, StarBright Yellow, and StarBright Red Dyes

StarBright Dyes: Improved Panel Design with New StarBright Blue, StarBright Yellow, and StarBright Red Dyes

A 27-color panel on the ZE5 Cell Analyzer demonstrates the use of the StarBright Dye range in a panel without the need for a special buffer. The benefits of using StarBright Yellow Dyes compared to PE-tandem dyes for multiplexing is also highlighted.
 

StarBright Dyes – Expanding the Size of Multicolor Spectral Panels with Superior Dyes Excitable by the UltraViolet, Violet, Blue, Yellow, and Red Lasers

StarBright Dyes: Expanding the Size of Multicolor Spectral Panels with Superior Dyes Excitable by the UltraViolet, Violet, Blue, Yellow, and Red Lasers

StarBright Dyes are ideal for use in spectral flow cytometry as shown in a large 43-color panel with a low complexity score. Unique spectral profiles of StarBright Yellow and StarBright Red Dyes enable the use of novel fluorophore combinations.
 

Rare Cell Population Detection with StarBright Dye Antibody Conjugates

Rare Cell Population Detection with StarBright Dye Antibody Conjugates

StarBright Dyes are bright, stable, and consistent making them a superior choice to detect and characterize rare cell populations, such as hematopoietic stem cells.
 

StarBright Red Dyes – Superior Dyes for Flow Cytometry

StarBright Red Dyes: Superior Dyes for Flow Cytometry

New StarBright Red Dyes are ideal for conventional and spectral flow cytometry. In addition, StarBright Dyes bound to tetramers via streptavidin can be used to identify antigen-specific T cells.
 


Spectral Unmixing to Substract Autofluoroscence in Samples Collected with the ZE5 Cell Analyzer

Spectral Unmixing to Substract Autofluoroscence in Samples Collected with the ZE5 Cell Analyzer

Spectral unmixing analysis and autofluorescence removal can improve population resolution over traditional compensation. For example, when studying pHrodo Green phagocytosis in J774 cells in combination with surface staining using StarBright Dye conjugated antibodies.
 




Expanding Immunophenotyping Panel Size with StarBright Blue and Yellow Dyes

Presented at CYTO 2022, these 21-color and 31-color, human immunophenotyping panels, showcase how the new StarBright Blue and StarBright Yellow Dyes can be used to expand both dye choice and panel size in immunophenotyping. Useful as a reference when building your own panels, see how these posters featuring StarBright Dyes in both conventional and multicolor flow cytometry.  Featuring data obtained using StarBright Dyes and the ZE5 Cell Analyzer and StarBright Dyes and a spectral flow cytometer, respectively. 

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Expanding Panel Size in Spectral Flow Cytometry with StarBright Dyes Excitable by the UltraViolet, Violet, and Blue Lasers.

Expanding Panel Size in Spectral Flow Cytometry with StarBright Dyes Excitable by the UltraViolet, Violet, and Blue Lasers

31-color spectral panel built using StarBright Dyes and a 4-laser spectral flow cytometer.
 

StarBright Dyes: Build Bigger, Better Panels With Superior Dyes Excitable by the UltraViolet, Violet, Blue, and Yellow Lasers.

StarBright Dyes: Build Bigger, Better Panels With Superior Dyes Excitable by the UltraViolet, Violet, Blue, and Yellow Lasers

21-color panel built using only antibodies from Bio-Rad and the ZE5 Cell Analyzer.
 




StarBright Violet, UltraViolet, Blue, and Yellow Dyes: Novel, Bright Fluorescent Dyes with Superior Capability

Presented at AAI 2022, these human immunophenotyping panels, showcase how StarBright Dyes can be used to expand panel size in multicolor immunophenotyping panels. Useful as a reference when building your own panels, see how these posters featuring StarBright Dyes and the ZE5 Cell Analyzer can help you build bigger better panels.

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StarBright Dyes – Build Bigger Better Panels with Superior Violet and Ultraviolet laser Excitable Dyes

StarBright Dyes – Build Bigger, Better Panels with Superior Violet and Ultraviolet Laser Excitable Dyes

Poster comparing the stain index of StarBright Dyes to dyes from other vendors, and the effect of pre-mixing antibody cocktails containing StarBright Violet and UltraViolet Dyes compared to freshly made up cocktails.
 

StarBright Dyes:- Build Bigger, Better Panels With Superior Dyes Excitable by the Blue, and Yellow lasers

StarBright Dyes:- Build Bigger, Better Panels With Superior Dyes Excitable by the Blue and Yellow Lasers.

11-color panels introducing StarBright Blue and StarBright Yellow Dyes on the ZE5 Cell Analyzer.
 




High-Parameter Immunophenotyping Panels Featuring StarBright Dyes

Presented at CYTO 2021, these 18-color and 27-color, human immunophenotyping panels, generated using StarBright Dyes and the ZE5 Cell Analyzer and a spectral flow cytometer respectively, highlight how to build successful panels while showcasing the properties and benefits of StarBright Dyes in large multicolor panel experiments.

Perfect as a reference when building your own panels.

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The Use of New StarBright Dyes in Spectral Flow Cytometry

The Use of New StarBright Dyes in Spectral Flow Cytometry

27-color spectral panel built using StarBright Dyes and a spectral flow cytometer.
 

Introducing StarBright Dyes, Novel Ultra Violet, Violet, and Blue Laser Excitable Fluorescent Nanoparticles Suitable for Immunophenotyping in Flow Cytometry

Introducing StarBright Dyes, Novel Ultra Violet, Violet, and Blue Laser Excitable Fluorescent Nanoparticles Suitable for Immunophenotyping in Flow Cytometry

18-color panel built using StarBright Dyes and the ZE5 Cell Analyzer.
 




StarBright Violet Dyes, Novel, Bright Fluorescent Nanoparticles Suitable for Flow Cytometry

Presented at AAI 2021, the two posters below highlight the incredible performance of StarBright Violet Dyes in conventional and spectral flow cytometry. These exceptionally bright dyes, with improved spectral properties, have better performance in multicolor panels and require no special buffer, compared to competitor dyes. Furthermore, they are photostable, fixable, and can be premixed for up to 28 days. Their unique spectra allow the use of novel dye combinations.

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StarBright Dyes — New Tools for Flow Cytometry

StarBright Dyes — New Tools for Flow Cytometry

The benefits of StarBright Dyes are highlighted in conventional and full spectrum flow cytometry.
 

Novel, Violet Fluorescent Nanoparticles for Immunophenotyping Using Flow Cytometry

Novel, Violet Fluorescent Nanoparticles for Immunophenotyping Using Flow Cytometry

Comparison of StarBright Dyes in multicolor immunophenotyping without the use of special buffers.