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CD25 antibody | AV142

Product Code Applications Pack Size List Price Your Price Qty
MCA5925GA
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SDS Safety Datasheet SDS
E F WB 0.1 mg loader
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loader

Mouse anti Chicken CD25 monoclonal antibody, clone AV142 recognizes CD25, the chicken IL-2 Receptor alpha chain (IL-2Rα) which has been identified as the chicken homologue of human CD25 and is cited as ChCD25 in published literature.
Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection .
Chicken IL-2Rα is the low affinity receptor for Interleukin-2.

Mouse anti Chicken CD25 monoclonal antibody, clone AV142 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to this anti chicken CD25 antibody, other antibodies to chicken CD antigens and cytokines from this project are now available from Bio-Rad.

Target Species
Chicken
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Chicken CD25/huIg fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 Ag14 Myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This reagent was produced by the WellcomeTrust-funded National Avian Resource Facility (Grant number WT099164MA).

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
ELISA
This product is suitable for use in direct ELISA applications.

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References for CD25 antibody

  1. Neulen, M.L. et al. (2015) Identification of novel chicken CD4+ CD3- blood population with NK cell like features.
    Dev Comp Immunol. 49 (1): 72-8.
  2. Geng, Y. et al. (2018) Dietary vitamin D(3) supplementation protects laying hens against lipopolysaccharide-induced immunological stress.
    Nutr Metab (Lond). 15: 58.
  3. Hohensee, L. et al. (2024) The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens.
    Vet Res. 55 (1): 5.
  4. Häätle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
    Frontiers in Veterinary Science. 11 [Epub ahead of print].

Synonyms
IL-2R Alpha Chain
UniProt
Q90WJ4

MCA5925GA

152082 156842 159855 164554

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