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CD279 antibody | EH12.2H7

Mouse anti Human CD279:FITC

Product Type
Monoclonal Antibody
Clone
EH12.2H7
Isotype
IgG1
Specificity
CD279

Product Code Applications Pack Size List Price Your Price Qty
MCA6133F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/400µl loader
List Price Your Price
loader

Mouse anti Human CD279 antibody, clone EH12.2H7 detects CD279, a co-stimulatory molecule also known as programmed cell death-1 (PD-1).

CD279 is a ~50-55 kDa membrane protein which is a member of the CD28 family, and functions as a regulator of T cell activation (Fife and Pauken, 2011). It has two specific ligands; CD274 (PD-L1) and CD273 (PD-L2), and their interaction is key in the balance between stimulatory and inhibitory signals needed for effective immune responses to microbes and self-tolerance.

CD279 is a marker for germinal center-associated T cells (Dorfman et al, 2006) and is inducibly expressed by T cells, B cells, NKT cells and monocytes upon activation.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Purity
>95% by SDS PAGE
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 4ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

Synonyms
PD-1
UniProt
Q15116
Entrez Gene
PDCD1
GO Terms
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0004725 protein tyrosine phosphatase activity
GO:0004871 signal transducer activity
GO:0006959 humoral immune response
GO:0007275 multicellular organismal development
GO:0031295 T cell costimulation

MCA6133F

149883 166096 166811

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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