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IgG antibody | MK 1 A6

Mouse anti Human IgG (Fc) CH2 Domain

Product Type
Monoclonal Antibody
Clone
MK 1 A6
Isotype
IgG1
Specificity
IgG
Region
(Fc) (CH2 DOMAIN)

Product Code Applications Pack Size List Price Your Price Qty
MCA647G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E WB 0.2 mg loader
List Price Your Price
loader

Mouse anti Human IgG (Fc) CH2 domain, clone MK 1 A6 recognizes human IgG Fc (all subclasses).

CH2 and hinge regions have an important role in effector functions of IgG. The epitope detected by clone MK 1 A6 lies within the CH2 domain as determined by haemagglutination and western blotting using IgG heavy chain and myelomas with defined domain deletions.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human IgG Polyclonal.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from BALB/c mouse were fused with cells from the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1,000 1/10,000
Immunohistology - Frozen 1
Immunohistology - Paraffin
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgG antibody

  1. Lund, J. et al. (1996) Multiple interactions of IgG with its core oligosaccharide can modulate recognition by complement and human Fc gamma receptor I and influence the synthesis of its oligosaccharide chains.
    J Immunol. 157 (11): 4963-9.
  2. Rasti, N. et al. (2006) Nonimmune immunoglobulin binding and multiple adhesion characterize Plasmodium falciparum-infected erythrocytes of placental origin.
    Proc Natl Acad Sci U S A. 103: 13795-800.
  3. Wozniak-Knopp, G. et al. (2010) Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties.
    Protein Eng Des Sel. 23: 289-97.
  4. Raghuraman, S. et al. (2012) Spontaneous clearance of chronic hepatitis C virus infection is associated with appearance of neutralizing antibodies and reversal of T-cell exhaustion.
    J Infect Dis. 205: 763-71.
  5. Hasenhindl, C. et al. (2013) Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.
    Protein Eng Des Sel. 26 (10): 675-82.
  6. Traxlmayr, M.W. et al. (2014) Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution.
    Biotechnol J. 9: 1013-22.
  7. Bali, N. et al. (2022) Sticks and Stones, a conserved cell surface ligand for the Type IIa RPTP Lar, regulates neural circuit wiring in Drosophila.
    Elife. 11: e71469.

ELISA

Flow Cytometry

RRID
AB_321910
UniProt
P01857
P01859
P01861
P01834
P01860
P0CG04
Entrez Gene
IGKC
IGHG1
IGHG2
IGHG3
IGHG4
IGLV2-14
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response

MCA647G

150235 158829 162460 164006

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