IgG2b antibody | AbD24127
![Anti Mouse IgG2b Antibody, clone AbD24127 thumbnail image 1](https://images.bio-rad-antibodies.com/monoclonal/mouse-igg2b-antibody-abd24127-hca311-image1-52w.jpg)
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![Anti Mouse IgG2b Antibody, clone AbD24127 thumbnail image 4](https://images.bio-rad-antibodies.com/monoclonal/mouse-igg2b-antibody-abd24127-hca311-image4-52w.jpg)
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![Anti Mouse IgG2b Antibody, clone AbD24127 thumbnail image 8](https://images.bio-rad-antibodies.com/monoclonal/mouse-igg2b-antibody-abd24127-hca311-image8-52w.jpg)
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Human anti Mouse IgG2b:DyLight®650
- Product Type
- Monoclonal Antibody
- Clone
- AbD24127
- Isotype
- HuCAL Fab bivalent
- Specificity
- IgG2b
Human anti mouse IgG2b monoclonal antibody, clone AbD24127, recognizes the IgG2b isotype of the primary antibody, with no species cross reactivity against mouse IgG1 and mouse IgG2a primary antibodies. This antibody allows for more than one unlabelled mouse monoclonal antibody to be used at any one time permitting multiplexing. |
- Target Species
- Mouse
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to DyLight 650 - liquid.
- Preparation
- Prepared by affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Dylight®650 654 673 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a trademark of EMD Biosciences.
DyLight is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/500 | 1/10,000 |
Immunocytochemistry | ![]() |
1/100 | 1/1000 |
Western Blotting | ![]() |
1/1000 | 1/10,000 |
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
HCA311D650
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If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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