CD19 antibody | LT19
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Mouse anti Human CD19:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- LT19
- Isotype
- IgG1
- Specificity
- CD19
Mouse anti Human CD19 antibody, clone LT19 recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rie et al. 1989). CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradbury et al. 1992), CD9, CD81 and CD82 (Horváth et al. 1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzutto et al. 1987). MCA1940SBV670 has been shown to recognize CD19 on mouse lymph node in live fluorescent IHC (Cook et al. 2024). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
Neat | 1/10 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 or 100μl whole blood
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
References for CD19 antibody
-
Hughes, G.J. et al. (2007) Virus immunocapture provides evidence of CD8 lymphocyte-derived HIV-1 in vivo.
AIDS. 21: 1507-13. -
Allen, J.S. et al. (2009) Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture.
Diabetes. 58: 138-45. -
McIntosh, K. et al. (2006) The immunogenicity of human adipose-derived cells: temporal changes in vitro.
Stem Cells. 24: 1246-53. -
Sengstake, S. et al. (2006) CD21 and CD62L shedding are both inducible via P2X7Rs.
Int Immunol. 18 (7): 1171-8. -
Villarroel Dorrego, M. et al. (2006) Transfection of CD40 in a human oral squamous cell carcinoma keratinocyte line upregulates immune potency and costimulatory molecules.
Br J Dermatol. 154: 231-8. -
Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
Blood. 118: 348-57. -
Lacal, P.M. et al. (2013) Glucocorticoid-induced tumor necrosis factor receptor family-related ligand triggering upregulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 and promotes leukocyte adhesion.
J Pharmacol Exp Ther. 347: 164-72. -
Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
Blood. 118: 348-57.
View The Latest Product References
-
Girbl, T. et al. (2013) CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility.
Cancer Res. 73: 561-70. -
Hertzberg, L. et al. (2010) Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group.
Blood. 115: 1006-17. -
Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.
Diab Vasc Dis Res. 8: 221-8. -
Dorvignit, D. et al. (2012) Expression and biological characterization of an anti-CD20 biosimilar candidate antibody: a case study.
MAbs. 4 (4): 488-96. -
Karlsen, M. et al. (2015) TLR-7 and -9 Stimulation of Peripheral Blood B Cells Indicate Altered TLR Signalling in Primary Sjögren's Syndrome Patients by Increased Secretion of Cytokines.
Scand J Immunol. 82 (6): 523-31. -
Clark, L.E. et al. (2018) Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses.
Nat Commun. 9 (1): 1884. -
Gu, Y. et al. (2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
Nat Commun. 10 (1): 893. -
Yang, C. et al. (2013) B cells promote tumor progression via STAT3 regulated-angiogenesis.
PLoS One. 8 (5): e64159. -
Cook, S.R. et al. (2024) Multi-organ immunity on a 3D-printed multi-tissue chip with tubing-free impeller pump.
bioRxiv. Jun 01 [Epub ahead of print].
- RRID
- AB_324759
- UniProt
- P15391
- Entrez Gene
- CD19
- GO Terms
- GO:0005515 protein binding
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0006968 cellular defense response
MCA1940A647
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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