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Mouse IgG2a Negative Control antibody

Mouse IgG2a Negative Control:Pacific Blue®

Product Type
Negative/Isotype Control
Isotype
IgG2a
Specificity
Mouse IgG2a Negative Control

Product Code Applications Pack Size List Price Your Price Qty
MCA929PB
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Mouse IgG2a negative control antibody, clone OX-34 is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.

Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.

This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.

Test results have shown that MCA929 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

This antibody may not be suitable for intracellular staining on some cell types.

Target Species
Negative Control
Product Form
Purified IgG conjugated to Pacific Blue® - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Activated rat T-helper cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Pacific Blue® 410 455
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
The Pacific Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagents.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for Mouse IgG2a Negative Control antibody

  1. Avigdor, A. et al. (2004) CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow.
    Blood. 103 (8): 2981-9.
  2. Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
    Avian Pathol. 45 (2): 235-43.
  3. Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
    Vet Res. 48 (1): 20.
  4. Terpeluk, R.E. et al. (2024) Supplementation of Foals with a Saccharomyces cerevisiae Fermentation Product Alters the Early Response to Vaccination
    Animals. 14 (6): 960.
  5. Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
    Vet Immunol Immunopathol. 254: 110520.
  6. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.

RRID
AB_567344

MCA929PB

150481 160238

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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