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HLA B7 antibody | BB7.1

Mouse anti Human HLA B7:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
BB7.1
Isotype
IgG1
Specificity
HLA B7

Product Code Applications Pack Size List Price Your Price Qty
MCA986A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
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Mouse anti Human HLA B7 antibody, clone BB7.1 recognizes the HLA B7 antigen and does not cross-react with HLA B27 or other related antigens. It can be used to distinguish true HLA B27 positives from false HLA B27 positives (i.e. HLA B7 positive) in the investigation of diseases such as ankylosing spondylitis and anterior uveitis. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C.

The HLA B gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Papain solubilized HLA-B7 antigen
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells or 100ul whole blood

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
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List Price Your Price
loader
Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for HLA B7 antibody

  1. Brodsky, F.M. et al. (1979) Monoclonal antibodies for analysis of the HLA system.
    Immunol Rev. 47: 3-61.
  2. Bonaparte, M.I. and Barker, E. (2004) Killing of human immunodeficiency virus-infected primary T-cell blasts by autologous natural killer cells is dependent on the ability of the virus to alter the expression of major histocompatibility complex class I molecules.
    Blood. 104: 2087-94.
  3. Anania, V.G. & Coscoy, L. (2011) Palmitoylation of MIR2 is required for its function.
    J Virol. 85 (5): 2288-95.
  4. Dellgren, C. et al. (2016) Low Constitutive Cell Surface Expression of HLA-B Is Caused by a Posttranslational Mechanism Involving Glu180 and Arg239.
    J Immunol. 197 (12): 4807-16.

Flow Cytometry

UniProt
P01889
Entrez Gene
HLA-B

MCA986A647

1806

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