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IgG antibody

Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(MOUSE ADSORBED)

Product Code Applications Pack Size List Price Your Price Qty
STAR71D650
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 0.1 mg loader
List Price Your Price
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Goat anti Rat IgG (mouse adsorbed) antibody recognizes rat IgG. Cross-reactivity with mouse IgG has been minimized by adsorption.

Target Species
Rat
Product Form
Purified IgG conjugated to DyLight®650 - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Rat IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Dylight®650 654 673
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/500
Immunofluorescence 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for IgG antibody

  1. Tamayo, J. et al. (2001) Chemical sensors and biosensors in liquid environment based on microcantilevers with amplified quality factor.
    Ultramicroscopy. 86: 167-73.
  2. Pérez-Bosque A et al. (2004) Dietary plasma protein affects the immune response of weaned rats challenged with S. aureus Superantigen B.
    J Nutr. 134: 2667-72.
  3. Balan, P. et al. (2010) Immunomodulatory effects of ovine serum immunoglobulin in the growing rat.
    Animal. 4: 1702-8.
  4. Yang, X. et al. (2010) The role of the JAK2-STAT3 pathway in pro-inflammatory responses of EMF-stimulated N9 microglial cells.
    J Neuroinflammation. 7: 54.
  5. Tulinská, J. et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
    Arch Toxicol. 92 (7): 2385-99.

UniProt
P20759
P20762
P20761
P20760
Entrez Gene
Ighg
LOC679045
LOC362795
GO Terms
GO:0003823 antigen binding

STAR71D650

152515

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products
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