5x ELISA Coating Buffer
5x ELISA Coating Buffer
- Product Type
- Accessory Reagent
- Specificity
- 5x ELISA Coating Buffer
- Intended Use
-
BUF030C: BUF030C is a proprietary pH 7.8 - 8.2 coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030C contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage. -
BUF030B: BUF030B is a proprietary coating buffer with pH7.8 - 8.2 for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030B contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage. -
BUF030A: BUF030A is a proprietary coating buffer with pH 7.8 - 8.2 for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage. - Product Form
- 5 x concentrate - liquid
- Preservative Stabilisers
- BUF030C, BUF030B: <0.5% sodium azide (NaN3)
-
BUF030A:
0.5% Sodium Azide - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Instructions For Use
- 1. Dilute the required volume of BUF030C 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation. - 1. Dilute the required volume of BUF030B 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation. - 1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
10x ELISA Wash Buffer | BUF031A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
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Description | 10x ELISA Wash Buffer | ||||||
ELISA BSA Block | BUF032A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
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Description | ELISA BSA Block |
References for 5x ELISA Coating Buffer
-
Böröcz, K. et al. (2022) Dynamic Features of Herd Immunity: Similarities in Age-Specific Anti-Measles Seroprevalence Data between Two Countries of Different Epidemiological History.
J Clin Med. 11 (4): 1145. -
Hayden, Z. et al. (2021) Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders.
J Immunol Res. 2021: 9953317. -
Szinger, D. et al. (2024) Raising Epidemiological Awareness: Assessment of Measles/MMR Susceptibility in Highly Vaccinated Clusters within the Hungarian and Croatian Population—A Sero-Surveillance Analysis
Vaccines. 12 (5): 486.
BUF030C
151991 154774 157898 159570 160379 162555 162863 163836 164522 166920 168752BUF030B
155121 156379 158381 158592 159767 163282 163890 164063 165543 167003 167620 168500BUF030A
154220 155413 156738 156978 157078 157468 157634 159477 162232 162345 163433 163925 165309 166990 167580If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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