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Antibodies for bioanalysis and drug monitoring of cemiplimab
Develop highly selective and sensitive pharmacokinetic (PK) and anti-drug antibody (ADA) assays for the monoclonal antibody drug cemiplimab (Libtayo) using our range of ready-made antibodies.
Table 1. Antibodies Specific to Cemiplimab.
Specificity |
Catalog Number |
Clone |
Format |
Affinity* KD, nM |
Assay Recommendation |
Product Details |
---|---|---|---|---|---|---|
Cemiplimab Inhibitory Type 1 |
TZA006 |
AbD41947ad |
Fab-F-Spy2-H1 |
2 |
PK bridging ELISA |
|
HCA367 |
AbD41949ia |
Human IgG1 |
6 |
ADA control |
||
HCA368 |
AbD41947ia |
Human IgG1 |
2 |
PK bridging ELISA ADA control |
||
HCA369 |
AbD41951ia |
Human IgG1 |
9 |
ADA control |
* Affinity measured in the monovalent Fab format.
1 Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags.
Type 1 anti-cemiplimab antibodies inhibit the binding of the drug cemiplimab to its target, human programmed death receptor-1 (PD-1). They are ideal for development of a PK bridging ELISA to measure free drug. In fully human IgG1 format, they are available in three affinities and are suitable as a surrogate positive control or calibrator for an ADA assay.
TrailBlazer Antibody, clone AbD41947ad (TZA006), has a SpyTag2 incorporated at the C-terminus of the antibody heavy chain. In a fast reaction, that simply requires mixing at room temperature, this antibody can form a covalent isopeptide bond to a SpyCatcher reagent, enabling site-directed conjugation or fast conversion to a bivalent Fab or a full-length Ig-like format within an hour.
Fig. 1. Cemiplimab PK bridging ELISA using antibodies TZA006 and HCA368.
Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.
Fig. 2. Cemiplimab ADA bridging ELISA using antibody HCA367, HCA368, or HCA369.
Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).
Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.