Proteus Endotoxin Removal MIDI Kits contain pre-packed affinity resin columns with extremely high endotoxin binding capacity, to achieve endotoxin removal effectively and quickly. These high performance kits result in more than 95% Endotoxin Removal in one passage, and greater than 90% Protein Recovery.
Proteus Endotoxin Removal MIDI Kits are available with different endotoxin binding capacities. Either kit can be used for samples with endotoxin loads less than 30,000 EU. For samples with endotoxin loads less than 1,000,000 EU, high capacity columns are required.
Kit | MIDI Standard Capacity | MIDI High Capacity | MINI columns |
---|---|---|---|
Typical binding capacity per column | 30,000 EU | 500,000 - 1,000,000 EU | 3,000 EU |
Typical endotoxin clearance 2 passes |
4 log reduction | 4 log reduction | N/A |
Typical endotoxin clearance (3 hour incubation) |
N/A | N/A | 3 log reduction |
Maximum sample load volume | 20 ml | 20 ml | 20 ml |
Bead size range | 100 µm | 100 µm | 100 µm |
Resin bed volume | 1 ml (pre-packed) | 1.7 ml (pre-packed) | 0.25 ml (loose resin) |
Recommended working pH | pH 4-8 | pH 4-8 | pH 4-8 |
Color coded end caps | Light green | Dark green | Supplied in vials |
Description | Centrifuge tubes |
High capacity |
Quantity | Product Code |
---|---|---|---|---|
Proteus Endotoxin Removal MIDI Spin Column Pack* | Yes | Yes | 12 Units | PUR028 |
Proteus Endotoxin Removal MIDI Spin Column Pack* | Yes | Yes | 12 Units | PUR030 |
Proteus Endotoxin Removal MINI Spin column Pack* | Yes | No | 12 Units | PUR032 |
Abbreviations: * Spin Column Packs contain the following: spin columns, spin column plugs, centrifuge tubes and plug insertion tools.
To answer any questions you may have about Proteus Kits, please consult our Proteus Purification Kit: FAQs Page.