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Develop highly selective and sensitive PK and ADA assays for adalimumab (Humira) using our range of ready-made anti-idiotypic antibodies.
Table 1. Antibodies Specific to Adalimumab and Adalimumab-TNFα Complex.
Specificity Binding Type |
Catalog Number |
Clone |
Format |
Affinity* KD, nM |
Assay Recommendation |
Product Details |
---|---|---|---|---|---|---|
Adalimumab Inhibitory Type 1 |
HCA202 |
AbD18654 |
Fab-FH1 |
0.15 |
PK bridging ELISA |
|
HCA203 |
AbD18654_hIgG1 |
hIgG1 |
0.15 |
ADA control |
||
HCA204 |
AbD18655_hIgG1 |
hIgG1 HRP |
0.06 |
PK bridging ELISA ADA control |
||
HCA271 |
AbD18655_hIgE |
hIgE |
0.06 |
ADA control |
||
HCA205 |
AbD18664_hIgG1 |
hIgG1 |
10.5 |
ADA control |
||
Adalimumab-TNFα Complex Specific Type 3 |
HCA206 |
AbD18754 |
Fab-FH1 |
67 |
PK ELISA antigen capture format |
|
HCA207 |
AbD18754_hIgG1 |
hIgG1 |
67 |
|||
HCA2312 |
AbD20350 |
Fab-FH1 |
3.2 |
|||
HCA2322 |
AbD20349_hIgG1 |
hIgG1 HRP |
6.7 |
* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags
2 HCA231 and HCA232/HCA232P are affinity matured versions of clone AbD18754 (HCA206 and HCA207, respectively)
Type 1 anti-adalimumab antibodies inhibit the binding of the drug adalimumab to its target, tumor necrosis factor alpha, TNFα. They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.
The Type 3 antibody specifically recognizes the drug-target complex, detecting adalimumab only when it is bound to TNFα. It is suitable as a detection antibody in a PK antigen capture assay.
Fig. 1. Adalimumab PK bridging ELISA using antibodies HCA202 and HCA204P.
Schematic image of PK bridging ELISA. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.
Fig. 2. Adalimumab PK antigen capture ELISA using antibody HCA231.
Schematic image of PK antigen capture ELISA. Drug target (red), monoclonal antibody drug (gold), drug target complex detection antibody, monovalent Fab format (purple), labeled with HRP.
Fig. 3. Adalimumab ADA bridging ELISA using antibodies HCA203, HCA204 and HCA205.
Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).
Fig. 4. Adalimumab IgE isotype ADA bridging ELISA using antibody HCA271.
Schematic image of ADA assay for IgE isotype detection. Monoclonal antibody drug as capture antibody (gold), fully human anti-idiotypic antibody, IgE format (blue), detection with HRP conjugated anti-human IgE antibody (gray).
Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.