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Develop highly selective and sensitive PK and ADA assays for the monoclonal antibody drug natalizumab (Tysabri) using our range of ready-made anti-idiotypic antibodies.
Table 1. Antibodies Specific to Natalizumab.
Specificity |
Catalog Number |
Clone |
Format |
Affinity* KD, nM |
Assay Recommendation |
Product Details |
---|---|---|---|---|---|---|
Natalizumab Inhibitory Type 1 |
HCA248 |
AbD21375 |
Fab-FH1 |
2.1 |
PK bridging ELISA |
|
HCA249 HCA249P |
AbD21375_hlgG1 |
Human IgG1 HRP |
2.1 |
PK bridging ELISA ADA control |
||
HCA250 |
AbD21384_hlgG1 |
Human IgG1 |
12.2 |
ADA control |
* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags
Type 1 anti-natalizumab antibodies inhibit the binding of the drug natalizumab to its target, human alpha4-beta1-integrin. They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.
Fig. 1. Natalizumab PK bridging ELISA using antibodies HCA248 and HCA249P.
Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.
Fig. 2. Natalizumab ADA bridging ELISA using antibody HCA249 or HCA250.
Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).
Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.